lambda ladder Search Results


94
New England Biolabs lambda ladder pfg marker
Lambda Ladder Pfg Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs lambda pfg ladder
PFGE profiles and dendrogram of the VAN susceptible VanB-type E. faecium isolates. Bacterial DNAs were digested with Sma I and separated by pulsed-field gel electrophoresis (PFGE). The genetic relatedness was analyzed using the Dice coefficient and the dendrogram was constructed with the clustering algorithm of Unweighted Pair-Group Method with an Arithmetic Mean (UPGMA) using FP Quest Software (Bio-Rad). The optimization and the tolerance were 1 and 1.5%, respectively. Major clusters and subclusters of the isolates were delineated with 85 and 90% similarity cutoff values for PFGE as indicated by the vertical solid line and dotted line, respectively. A <t>lambda</t> <t>PFG</t> Ladder (New England BioLabs, MA) was used as the Molecular Marker (MM)
Lambda Pfg Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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90
Boehringer Mannheim bacteriophage lambda ladder pfge markers
<t>PFGE</t> fingerprint patterns of S. suis isolates used in the present study using the ApaI endonuclease. Lanes 1 to 6, fluoroquinolone-susceptible strains; lanes 7 to 17, fluoroquinolone-resistant isolates. The first and last lanes are <t>bacteriophage</t> <t>lambda</t> ladder PFGE markers (Boehringer Mannheim, Germany); lane 1, S. suis ATCC 43765; lane 2, BB1001; lane 3, BB1002; lane 4, BB1003; lane 5, BB1004; lane 6, BB1005; lane 7, BB1006; lane 8, BB1007; lane 9, BB1008; lane 10, BB1009; lane 11, BB1011; lane 12, BB1012; lane 13, BB1013; lane 14, BB1014; lane 15, BB1015; lane 16, BB1016; lane 17, BB1017. Analysis was performed with QuantityOne software (Bio-Rad, Richmond, CA).
Bacteriophage Lambda Ladder Pfge Markers, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Boehringer Mannheim bacteriophage lambda ladder pfge marker
<t>PFGE</t> fingerprint patterns of S. suis isolates used in the present study using the ApaI endonuclease. Lanes 1 to 6, fluoroquinolone-susceptible strains; lanes 7 to 17, fluoroquinolone-resistant isolates. The first and last lanes are <t>bacteriophage</t> <t>lambda</t> ladder PFGE markers (Boehringer Mannheim, Germany); lane 1, S. suis ATCC 43765; lane 2, BB1001; lane 3, BB1002; lane 4, BB1003; lane 5, BB1004; lane 6, BB1005; lane 7, BB1006; lane 8, BB1007; lane 9, BB1008; lane 10, BB1009; lane 11, BB1011; lane 12, BB1012; lane 13, BB1013; lane 14, BB1014; lane 15, BB1015; lane 16, BB1016; lane 17, BB1017. Analysis was performed with QuantityOne software (Bio-Rad, Richmond, CA).
Bacteriophage Lambda Ladder Pfge Marker, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bacteriophage lambda ladder pfge marker/product/Boehringer Mannheim
Average 90 stars, based on 1 article reviews
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Promega promega-markers lambda ladders
<t>PFGE</t> fingerprint patterns of S. suis isolates used in the present study using the ApaI endonuclease. Lanes 1 to 6, fluoroquinolone-susceptible strains; lanes 7 to 17, fluoroquinolone-resistant isolates. The first and last lanes are <t>bacteriophage</t> <t>lambda</t> ladder PFGE markers (Boehringer Mannheim, Germany); lane 1, S. suis ATCC 43765; lane 2, BB1001; lane 3, BB1002; lane 4, BB1003; lane 5, BB1004; lane 6, BB1005; lane 7, BB1006; lane 8, BB1007; lane 9, BB1008; lane 10, BB1009; lane 11, BB1011; lane 12, BB1012; lane 13, BB1013; lane 14, BB1014; lane 15, BB1015; lane 16, BB1016; lane 17, BB1017. Analysis was performed with QuantityOne software (Bio-Rad, Richmond, CA).
Promega Markers Lambda Ladders, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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promega-markers lambda ladders - by Bioz Stars, 2026-02
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Promega lambda ladder promega
<t>PFGE</t> fingerprint patterns of S. suis isolates used in the present study using the ApaI endonuclease. Lanes 1 to 6, fluoroquinolone-susceptible strains; lanes 7 to 17, fluoroquinolone-resistant isolates. The first and last lanes are <t>bacteriophage</t> <t>lambda</t> ladder PFGE markers (Boehringer Mannheim, Germany); lane 1, S. suis ATCC 43765; lane 2, BB1001; lane 3, BB1002; lane 4, BB1003; lane 5, BB1004; lane 6, BB1005; lane 7, BB1006; lane 8, BB1007; lane 9, BB1008; lane 10, BB1009; lane 11, BB1011; lane 12, BB1012; lane 13, BB1013; lane 14, BB1014; lane 15, BB1015; lane 16, BB1016; lane 17, BB1017. Analysis was performed with QuantityOne software (Bio-Rad, Richmond, CA).
Lambda Ladder Promega, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega λ ladders
a Three types of GCRs (translocation, isochromosome formation, and truncation) can be detected using ChL C . The three can be differentiated by length. b Chromosomal DNAs from parental strains (P) and independent GCR clones of wild type, rad51 ∆, rad52 ∆, and rad52 ∆ rad51 ∆ were separated by broad- and short-range PFGE and stained with EtBr (top and bottom rows, respectively). Positions of chr1, chr2, chr3, and ChL C (5.7, 4.6, ~3.5, and 0.5 Mb, respectively) in the parental strains are indicated on the left side of the broad-range PFGE panels (top row). Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of <t>λ</t> <t>ladders</t> (Promega). c PCR analysis of GCR products recovered from agarose gels. Both sides of cnt3–imr3 junctions were amplified and resolved by standard agarose gel electrophoresis (cnt3–imr3). Amplified irc3R and irc3L regions were digested with ApoI (irc3L & irc3R) prior to the electrophoresis. Positions of primers (red arrows) and ApoI sites are indicated at the bottom. A, ApoI. d Rates of truncation (magenta) and isochromosome formation (black). Rates relative to that of the rad51 ∆ strain are indicated. Uncropped images of the gels presented in b , c are shown in Supplementary Fig. . Source data for the graph in d are available in Supplementary Data .
λ Ladders, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Lonza a lambda dna ladder (48.5 kb–1 mb)
a Three types of GCRs (translocation, isochromosome formation, and truncation) can be detected using ChL C . The three can be differentiated by length. b Chromosomal DNAs from parental strains (P) and independent GCR clones of wild type, rad51 ∆, rad52 ∆, and rad52 ∆ rad51 ∆ were separated by broad- and short-range PFGE and stained with EtBr (top and bottom rows, respectively). Positions of chr1, chr2, chr3, and ChL C (5.7, 4.6, ~3.5, and 0.5 Mb, respectively) in the parental strains are indicated on the left side of the broad-range PFGE panels (top row). Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of <t>λ</t> <t>ladders</t> (Promega). c PCR analysis of GCR products recovered from agarose gels. Both sides of cnt3–imr3 junctions were amplified and resolved by standard agarose gel electrophoresis (cnt3–imr3). Amplified irc3R and irc3L regions were digested with ApoI (irc3L & irc3R) prior to the electrophoresis. Positions of primers (red arrows) and ApoI sites are indicated at the bottom. A, ApoI. d Rates of truncation (magenta) and isochromosome formation (black). Rates relative to that of the rad51 ∆ strain are indicated. Uncropped images of the gels presented in b , c are shown in Supplementary Fig. . Source data for the graph in d are available in Supplementary Data .
A Lambda Dna Ladder (48.5 Kb–1 Mb), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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90
Promega markers lambda ladders
a Three types of GCRs (translocation, isochromosome formation, and truncation) can be detected using ChL C . The three can be differentiated by length. b Chromosomal DNAs from parental strains (P) and independent GCR clones of wild type, rad51 ∆, rad52 ∆, and rad52 ∆ rad51 ∆ were separated by broad- and short-range PFGE and stained with EtBr (top and bottom rows, respectively). Positions of chr1, chr2, chr3, and ChL C (5.7, 4.6, ~3.5, and 0.5 Mb, respectively) in the parental strains are indicated on the left side of the broad-range PFGE panels (top row). Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of <t>λ</t> <t>ladders</t> (Promega). c PCR analysis of GCR products recovered from agarose gels. Both sides of cnt3–imr3 junctions were amplified and resolved by standard agarose gel electrophoresis (cnt3–imr3). Amplified irc3R and irc3L regions were digested with ApoI (irc3L & irc3R) prior to the electrophoresis. Positions of primers (red arrows) and ApoI sites are indicated at the bottom. A, ApoI. d Rates of truncation (magenta) and isochromosome formation (black). Rates relative to that of the rad51 ∆ strain are indicated. Uncropped images of the gels presented in b , c are shown in Supplementary Fig. . Source data for the graph in d are available in Supplementary Data .
Markers Lambda Ladders, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega promega-markers® lambda ladders marker
a Three types of GCRs (translocation, isochromosome formation, and truncation) can be detected using ChL C . The three can be differentiated by length. b Chromosomal DNAs from parental strains (P) and independent GCR clones of wild type, rad51 ∆, rad52 ∆, and rad52 ∆ rad51 ∆ were separated by broad- and short-range PFGE and stained with EtBr (top and bottom rows, respectively). Positions of chr1, chr2, chr3, and ChL C (5.7, 4.6, ~3.5, and 0.5 Mb, respectively) in the parental strains are indicated on the left side of the broad-range PFGE panels (top row). Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of <t>λ</t> <t>ladders</t> (Promega). c PCR analysis of GCR products recovered from agarose gels. Both sides of cnt3–imr3 junctions were amplified and resolved by standard agarose gel electrophoresis (cnt3–imr3). Amplified irc3R and irc3L regions were digested with ApoI (irc3L & irc3R) prior to the electrophoresis. Positions of primers (red arrows) and ApoI sites are indicated at the bottom. A, ApoI. d Rates of truncation (magenta) and isochromosome formation (black). Rates relative to that of the rad51 ∆ strain are indicated. Uncropped images of the gels presented in b , c are shown in Supplementary Fig. . Source data for the graph in d are available in Supplementary Data .
Promega Markers® Lambda Ladders Marker, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Intact Genomics lambda dna ladder marker intact genomics
a Three types of GCRs (translocation, isochromosome formation, and truncation) can be detected using ChL C . The three can be differentiated by length. b Chromosomal DNAs from parental strains (P) and independent GCR clones of wild type, rad51 ∆, rad52 ∆, and rad52 ∆ rad51 ∆ were separated by broad- and short-range PFGE and stained with EtBr (top and bottom rows, respectively). Positions of chr1, chr2, chr3, and ChL C (5.7, 4.6, ~3.5, and 0.5 Mb, respectively) in the parental strains are indicated on the left side of the broad-range PFGE panels (top row). Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of <t>λ</t> <t>ladders</t> (Promega). c PCR analysis of GCR products recovered from agarose gels. Both sides of cnt3–imr3 junctions were amplified and resolved by standard agarose gel electrophoresis (cnt3–imr3). Amplified irc3R and irc3L regions were digested with ApoI (irc3L & irc3R) prior to the electrophoresis. Positions of primers (red arrows) and ApoI sites are indicated at the bottom. A, ApoI. d Rates of truncation (magenta) and isochromosome formation (black). Rates relative to that of the rad51 ∆ strain are indicated. Uncropped images of the gels presented in b , c are shown in Supplementary Fig. . Source data for the graph in d are available in Supplementary Data .
Lambda Dna Ladder Marker Intact Genomics, supplied by Intact Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega lambda dna/hindiii dna ladder
a Three types of GCRs (translocation, isochromosome formation, and truncation) can be detected using ChL C . The three can be differentiated by length. b Chromosomal DNAs from parental strains (P) and independent GCR clones of wild type, rad51 ∆, rad52 ∆, and rad52 ∆ rad51 ∆ were separated by broad- and short-range PFGE and stained with EtBr (top and bottom rows, respectively). Positions of chr1, chr2, chr3, and ChL C (5.7, 4.6, ~3.5, and 0.5 Mb, respectively) in the parental strains are indicated on the left side of the broad-range PFGE panels (top row). Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of <t>λ</t> <t>ladders</t> (Promega). c PCR analysis of GCR products recovered from agarose gels. Both sides of cnt3–imr3 junctions were amplified and resolved by standard agarose gel electrophoresis (cnt3–imr3). Amplified irc3R and irc3L regions were digested with ApoI (irc3L & irc3R) prior to the electrophoresis. Positions of primers (red arrows) and ApoI sites are indicated at the bottom. A, ApoI. d Rates of truncation (magenta) and isochromosome formation (black). Rates relative to that of the rad51 ∆ strain are indicated. Uncropped images of the gels presented in b , c are shown in Supplementary Fig. . Source data for the graph in d are available in Supplementary Data .
Lambda Dna/Hindiii Dna Ladder, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


PFGE profiles and dendrogram of the VAN susceptible VanB-type E. faecium isolates. Bacterial DNAs were digested with Sma I and separated by pulsed-field gel electrophoresis (PFGE). The genetic relatedness was analyzed using the Dice coefficient and the dendrogram was constructed with the clustering algorithm of Unweighted Pair-Group Method with an Arithmetic Mean (UPGMA) using FP Quest Software (Bio-Rad). The optimization and the tolerance were 1 and 1.5%, respectively. Major clusters and subclusters of the isolates were delineated with 85 and 90% similarity cutoff values for PFGE as indicated by the vertical solid line and dotted line, respectively. A lambda PFG Ladder (New England BioLabs, MA) was used as the Molecular Marker (MM)

Journal: BMC Microbiology

Article Title: Dissemination and genetic analysis of the stealthy vanB gene clusters of Enterococcus faecium clinical isolates in Japan

doi: 10.1186/s12866-018-1342-1

Figure Lengend Snippet: PFGE profiles and dendrogram of the VAN susceptible VanB-type E. faecium isolates. Bacterial DNAs were digested with Sma I and separated by pulsed-field gel electrophoresis (PFGE). The genetic relatedness was analyzed using the Dice coefficient and the dendrogram was constructed with the clustering algorithm of Unweighted Pair-Group Method with an Arithmetic Mean (UPGMA) using FP Quest Software (Bio-Rad). The optimization and the tolerance were 1 and 1.5%, respectively. Major clusters and subclusters of the isolates were delineated with 85 and 90% similarity cutoff values for PFGE as indicated by the vertical solid line and dotted line, respectively. A lambda PFG Ladder (New England BioLabs, MA) was used as the Molecular Marker (MM)

Article Snippet: A lambda PFG Ladder (New England BioLabs, MA) was used as the Molecular Marker (MM)

Techniques: Pulsed-Field Gel, Electrophoresis, Construct, Software, Marker

PFGE fingerprint patterns of S. suis isolates used in the present study using the ApaI endonuclease. Lanes 1 to 6, fluoroquinolone-susceptible strains; lanes 7 to 17, fluoroquinolone-resistant isolates. The first and last lanes are bacteriophage lambda ladder PFGE markers (Boehringer Mannheim, Germany); lane 1, S. suis ATCC 43765; lane 2, BB1001; lane 3, BB1002; lane 4, BB1003; lane 5, BB1004; lane 6, BB1005; lane 7, BB1006; lane 8, BB1007; lane 9, BB1008; lane 10, BB1009; lane 11, BB1011; lane 12, BB1012; lane 13, BB1013; lane 14, BB1014; lane 15, BB1015; lane 16, BB1016; lane 17, BB1017. Analysis was performed with QuantityOne software (Bio-Rad, Richmond, CA).

Journal:

Article Title: First Characterization of Fluoroquinolone Resistance in Streptococcus suis

doi: 10.1128/AAC.00972-06

Figure Lengend Snippet: PFGE fingerprint patterns of S. suis isolates used in the present study using the ApaI endonuclease. Lanes 1 to 6, fluoroquinolone-susceptible strains; lanes 7 to 17, fluoroquinolone-resistant isolates. The first and last lanes are bacteriophage lambda ladder PFGE markers (Boehringer Mannheim, Germany); lane 1, S. suis ATCC 43765; lane 2, BB1001; lane 3, BB1002; lane 4, BB1003; lane 5, BB1004; lane 6, BB1005; lane 7, BB1006; lane 8, BB1007; lane 9, BB1008; lane 10, BB1009; lane 11, BB1011; lane 12, BB1012; lane 13, BB1013; lane 14, BB1014; lane 15, BB1015; lane 16, BB1016; lane 17, BB1017. Analysis was performed with QuantityOne software (Bio-Rad, Richmond, CA).

Article Snippet: The first and last lanes are bacteriophage lambda ladder PFGE markers (Boehringer Mannheim, Germany); lane 1, S. suis ATCC 43765; lane 2, BB1001; lane 3, BB1002; lane 4, BB1003; lane 5, BB1004; lane 6, BB1005; lane 7, BB1006; lane 8, BB1007; lane 9, BB1008; lane 10, BB1009; lane 11, BB1011; lane 12, BB1012; lane 13, BB1013; lane 14, BB1014; lane 15, BB1015; lane 16, BB1016; lane 17, BB1017.

Techniques: Software

a Three types of GCRs (translocation, isochromosome formation, and truncation) can be detected using ChL C . The three can be differentiated by length. b Chromosomal DNAs from parental strains (P) and independent GCR clones of wild type, rad51 ∆, rad52 ∆, and rad52 ∆ rad51 ∆ were separated by broad- and short-range PFGE and stained with EtBr (top and bottom rows, respectively). Positions of chr1, chr2, chr3, and ChL C (5.7, 4.6, ~3.5, and 0.5 Mb, respectively) in the parental strains are indicated on the left side of the broad-range PFGE panels (top row). Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of λ ladders (Promega). c PCR analysis of GCR products recovered from agarose gels. Both sides of cnt3–imr3 junctions were amplified and resolved by standard agarose gel electrophoresis (cnt3–imr3). Amplified irc3R and irc3L regions were digested with ApoI (irc3L & irc3R) prior to the electrophoresis. Positions of primers (red arrows) and ApoI sites are indicated at the bottom. A, ApoI. d Rates of truncation (magenta) and isochromosome formation (black). Rates relative to that of the rad51 ∆ strain are indicated. Uncropped images of the gels presented in b , c are shown in Supplementary Fig. . Source data for the graph in d are available in Supplementary Data .

Journal: Communications Biology

Article Title: DNA replication machinery prevents Rad52-dependent single-strand annealing that leads to gross chromosomal rearrangements at centromeres

doi: 10.1038/s42003-020-0934-0

Figure Lengend Snippet: a Three types of GCRs (translocation, isochromosome formation, and truncation) can be detected using ChL C . The three can be differentiated by length. b Chromosomal DNAs from parental strains (P) and independent GCR clones of wild type, rad51 ∆, rad52 ∆, and rad52 ∆ rad51 ∆ were separated by broad- and short-range PFGE and stained with EtBr (top and bottom rows, respectively). Positions of chr1, chr2, chr3, and ChL C (5.7, 4.6, ~3.5, and 0.5 Mb, respectively) in the parental strains are indicated on the left side of the broad-range PFGE panels (top row). Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of λ ladders (Promega). c PCR analysis of GCR products recovered from agarose gels. Both sides of cnt3–imr3 junctions were amplified and resolved by standard agarose gel electrophoresis (cnt3–imr3). Amplified irc3R and irc3L regions were digested with ApoI (irc3L & irc3R) prior to the electrophoresis. Positions of primers (red arrows) and ApoI sites are indicated at the bottom. A, ApoI. d Rates of truncation (magenta) and isochromosome formation (black). Rates relative to that of the rad51 ∆ strain are indicated. Uncropped images of the gels presented in b , c are shown in Supplementary Fig. . Source data for the graph in d are available in Supplementary Data .

Article Snippet: Numbers on the left of the short-range PFGE panels (bottom row) indicate sizes of λ ladders (Promega). c PCR analysis of GCR products recovered from agarose gels.

Techniques: Translocation Assay, Clone Assay, Staining, Amplification, Agarose Gel Electrophoresis, Electrophoresis